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Research chemical Published: human trials Established use

SS-31: dosing, reconstitution and evidence

A mitochondria-targeting tetrapeptide that binds cardiolipin in the inner mitochondrial membrane, improving efficiency and cutting oxidative stress. Has reached Phase 3 for primary mitochondrial myopathy.

Also known as Elamipretide, Bendavia, MTP-131

Educational research reference Longevity & Cellular
Quick reference. Everything you need to run SS-31 — dosing, frequency, reconstitution math for the vial sizes people actually buy, and what it stacks with. For anything specific to your situation, ask the Guru.

SS-31 quick start

Route
SubQ
Typical dose
5–20 mg
Frequency
Daily
Half-life
Roughly 2–4 hours.
Cycle
Trials ran continuously; independent use typically 4–12 weeks.
Vial sizes sold
50mg or 10mg
These are the numbers people actually run, drawn from the published record and from what the community has settled on over time. Educational reference, not medical advice — a starting point to work from, and one you can adjust as you learn how you respond.

How to run it

How often
Daily
Cycle length
Trials ran continuously; independent use typically 4–12 weeks.
Half-life
Roughly 2–4 hours.
Route
SubQ
Consistency matters more than perfect timing. Pick a slot in your day you will actually keep, and let DoseIQ handle the rest.

What people actually run

Used by the longevity crowd. Expensive and frequently counterfeited, so sourcing matters more than usual.

This is accumulated community practice — years of real-world use across a large number of people, converging on numbers that work. It is not a controlled trial, and for most compounds here no such trial exists or is ever likely to. That makes this the most reliable dosing signal available, and it is a good deal better than guessing.

What to expect

Most people tolerate SS-31 well. Here is what is worth knowing going in, so nothing catches you by surprise.

Better characterized than most here — real Phase 2/3 human trials — but still unapproved. Injection site reactions are the most common trial finding. Expensive and frequently counterfeited.

Reconstitution guide

SS-31 ships as a lyophilised powder and gets mixed with bacteriostatic water before use. How much water you add sets the concentration, which sets how many syringe units make up each dose. One unit on a U-100 syringe is 0.01 mL — the tables below do the arithmetic for the vial sizes you are most likely to be holding.

50mg vial most common

Water addedConcentration 5mg20mg
2mL (common) 25 mg/mL0.20 mL
20 units		0.80 mL
80 units
3mL 16.67 mg/mL0.30 mL
30 units		1.20 mL
120 units *

10mg vial

Water addedConcentration 5mg20mg
2mL (common) 5 mg/mL1.00 mL
100 units		4.00 mL
400 units *
3mL 3.33 mg/mL1.50 mL
150 units *		6.00 mL
600 units *

* More than 100 units — that is more than a single U-100 insulin syringe holds. Either use a larger syringe, split it across two draws, or mix with less water to concentrate it. Adding less water is usually the easier fix.

50 mg vial + 1 mL BAC water = 50 mg/mL — a 10 mg dose = 20 units on a U-100 syringe. 10 mg vial + 1 mL = 10 mg/mL — 10 mg = 100 units.

Standard steps

  1. Inspect the vialConfirm the label and that the powder cake is intact. Do not use a cracked or compromised vial.
  2. Let it reach room temperatureSitting out for a few minutes reduces condensation inside the vial when the seal is broken.
  3. Disinfect both stoppersWipe the compound vial and the bacteriostatic water vial with a fresh alcohol swab and let them air-dry.
  4. Draw the bacteriostatic waterUse a sterile syringe to draw the volume you have chosen from the reconstitution table.
  5. Add the water down the vial wallInject slowly so it runs down the inside wall rather than spraying directly onto the powder. This limits foaming.
  6. Dissolve gentlySwirl or roll the vial between your hands until the cake dissolves. Never shake — agitation damages peptide bonds. The solution should end up clear.
  7. Label and refrigerateWrite the reconstitution date and concentration on the vial. Store at 2–8°C. Do not freeze.
Running a different vial size or water volume? The DoseIQ calculator works it out for any combination — mcg, mg or IU, on any syringe size.
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This page is an educational research reference and is not medical advice. SS-31 is not approved for human use in any jurisdiction. Dose figures reflect the published record and what the community has converged on — starting points to weigh, not instructions. Consult a qualified healthcare provider before starting any protocol.