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Over the counter Published: human trials Extensive real-world use

NAD+: dosing, reconstitution and evidence

A coenzyme central to mitochondrial energy production and DNA repair. Levels fall with age. Used for energy, cognitive clarity and cellular repair.

Also known as Nicotinamide Adenine Dinucleotide

Educational research reference Longevity & Cellular
Quick reference. Everything you need to run NAD+ — dosing, frequency, reconstitution math for the vial sizes people actually buy, and what it stacks with. For anything specific to your situation, ask the Guru.

NAD+ quick start

Route
SubQ, IV or intranasal
Typical dose
50–100 mg SubQ; 250–1,000 mg IV
Frequency
Daily to weekly
Half-life
Rapid clearance — minutes. IV infusions are given slowly for this reason.
Cycle
Continuous or in cycles.
Vial sizes sold
500mg or 1000mg
These are the numbers people actually run, drawn from the published record and from what the community has settled on over time. Educational reference, not medical advice — a starting point to work from, and one you can adjust as you learn how you respond.

How to run it

How often
Daily to weekly
Cycle length
Continuous or in cycles.
Half-life
Rapid clearance — minutes. IV infusions are given slowly for this reason.
Route
SubQ, IV or intranasal
Consistency matters more than perfect timing. Pick a slot in your day you will actually keep, and let DoseIQ handle the rest.

What people actually run

Very widely used. Community knows the SubQ sting is expected and that slow IV is non-negotiable.

This is accumulated community practice — years of real-world use across a large number of people, converging on numbers that work. It is not a controlled trial, and for most compounds here no such trial exists or is ever likely to. That makes this the most reliable dosing signal available, and it is a good deal better than guessing.

What to expect

Most people tolerate NAD+ well. Here is what is worth knowing going in, so nothing catches you by surprise.

SubQ injection is notably painful and often causes a burning sensation — this is expected. Rapid IV infusion causes chest tightness, nausea and flushing; infusions must be slow. Human evidence for oral precursors is stronger than for direct NAD+ injection.

Reconstitution guide

NAD+ ships as a lyophilised powder and gets mixed with bacteriostatic water before use. How much water you add sets the concentration, which sets how many syringe units make up each dose. One unit on a U-100 syringe is 0.01 mL — the tables below do the arithmetic for the vial sizes you are most likely to be holding.

500mg vial most common

Water addedConcentration 50mg100mg
5mL (common) 100 mg/mL0.50 mL
50 units		1.00 mL
100 units
10mL 50 mg/mL1.00 mL
100 units		2.00 mL
200 units *

1000mg vial

Water addedConcentration 50mg100mg
5mL (common) 200 mg/mL0.25 mL
25 units		0.50 mL
50 units
10mL 100 mg/mL0.50 mL
50 units		1.00 mL
100 units

* More than 100 units — that is more than a single U-100 insulin syringe holds. Either use a larger syringe, split it across two draws, or mix with less water to concentrate it. Adding less water is usually the easier fix.

500 mg vial + 5 mL BAC water = 100 mg/mL — a 100 mg dose = 100 units on a U-100 syringe. 1000 mg vial + 10 mL = 100 mg/mL — 100 mg = 100 units.

Standard steps

  1. Inspect the vialConfirm the label and that the powder cake is intact. Do not use a cracked or compromised vial.
  2. Let it reach room temperatureSitting out for a few minutes reduces condensation inside the vial when the seal is broken.
  3. Disinfect both stoppersWipe the compound vial and the bacteriostatic water vial with a fresh alcohol swab and let them air-dry.
  4. Draw the bacteriostatic waterUse a sterile syringe to draw the volume you have chosen from the reconstitution table.
  5. Add the water down the vial wallInject slowly so it runs down the inside wall rather than spraying directly onto the powder. This limits foaming.
  6. Dissolve gentlySwirl or roll the vial between your hands until the cake dissolves. Never shake — agitation damages peptide bonds. The solution should end up clear.
  7. Label and refrigerateWrite the reconstitution date and concentration on the vial. Store at 2–8°C. Do not freeze.
Running a different vial size or water volume? The DoseIQ calculator works it out for any combination — mcg, mg or IU, on any syringe size.
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This page is an educational research reference and is not medical advice. Dose figures reflect the published record and what the community has converged on — starting points to weigh, not instructions. Consult a qualified healthcare provider before starting any protocol.