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Research chemical Published: animal data only Emerging use

FOXO4-DRI: dosing, reconstitution and evidence

A senolytic peptide designed to break the FOXO4–p53 interaction that keeps senescent cells alive, prompting them to self-destruct. The mouse data on restoring fur density and kidney function drove most of the interest.

Also known as FOXO4-D-Retro-Inverso, senolytic peptide

Educational research reference Longevity & Cellular
Quick reference. Everything you need to run FOXO4-DRI — dosing, frequency, reconstitution math for the vial sizes people actually buy, and what it stacks with. For anything specific to your situation, ask the Guru.

FOXO4-DRI quick start

Route
SubQ or IV
Typical dose
5–10 mg per dose
Frequency
3 doses over one week, then months off
Half-life
Short. The pulsed protocol is the point — senolytics are meant to clear cells and stop, not to be present continuously.
Cycle
One 3-dose week, then 3–6 months off.
Vial sizes sold
10mg or 5mg
These are the numbers people actually run, drawn from the published record and from what the community has settled on over time. Educational reference, not medical advice — a starting point to work from, and one you can adjust as you learn how you respond.

How to run it

How often
3 doses over one week, then months off
Cycle length
One 3-dose week, then 3–6 months off.
Half-life
Short. The pulsed protocol is the point — senolytics are meant to clear cells and stop, not to be present continuously.
Route
SubQ or IV
Consistency matters more than perfect timing. Pick a slot in your day you will actually keep, and let DoseIQ handle the rest.

What people actually run

Expensive and used in short pulses rather than continuously — three doses over a week, then months off. Among the most speculative things people run.

This is accumulated community practice — years of real-world use across a large number of people, converging on numbers that work. It is not a controlled trial, and for most compounds here no such trial exists or is ever likely to. That makes this the most reliable dosing signal available, and it is a good deal better than guessing.

What to expect

Most people tolerate FOXO4-DRI well. Here is what is worth knowing going in, so nothing catches you by surprise.

The most speculative compound in this library, and priced accordingly. Killing senescent cells is not free — some are there for wound healing and tissue structure, and clearing them indiscriminately has consequences nobody has measured in humans. Flu-like symptoms for a day or two after dosing are commonly reported. No human trials of any kind. Pulsed dosing is not a preference, it is how the compound is meant to work.

Reconstitution guide

FOXO4-DRI ships as a lyophilised powder and gets mixed with bacteriostatic water before use. How much water you add sets the concentration, which sets how many syringe units make up each dose. One unit on a U-100 syringe is 0.01 mL — the tables below do the arithmetic for the vial sizes you are most likely to be holding.

10mg vial most common

Water addedConcentration 5mg10mg
2mL (common) 5 mg/mL1.00 mL
100 units		2.00 mL
200 units *
3mL 3.33 mg/mL1.50 mL
150 units *		3.00 mL
300 units *

5mg vial

Water addedConcentration 5mg10mg
2mL (common) 2.5 mg/mL2.00 mL
200 units *		4.00 mL
400 units *
3mL 1.67 mg/mL3.00 mL
300 units *		6.00 mL
600 units *

* More than 100 units — that is more than a single U-100 insulin syringe holds. Either use a larger syringe, split it across two draws, or mix with less water to concentrate it. Adding less water is usually the easier fix.

10 mg vial + 1 mL BAC water = 10 mg/mL — a 5 mg dose = 50 units on a U-100 syringe. 5 mg vial + 1 mL = 5 mg/mL — 5 mg = 100 units.

Standard steps

  1. Inspect the vialConfirm the label and that the powder cake is intact. Do not use a cracked or compromised vial.
  2. Let it reach room temperatureSitting out for a few minutes reduces condensation inside the vial when the seal is broken.
  3. Disinfect both stoppersWipe the compound vial and the bacteriostatic water vial with a fresh alcohol swab and let them air-dry.
  4. Draw the bacteriostatic waterUse a sterile syringe to draw the volume you have chosen from the reconstitution table.
  5. Add the water down the vial wallInject slowly so it runs down the inside wall rather than spraying directly onto the powder. This limits foaming.
  6. Dissolve gentlySwirl or roll the vial between your hands until the cake dissolves. Never shake — agitation damages peptide bonds. The solution should end up clear.
  7. Label and refrigerateWrite the reconstitution date and concentration on the vial. Store at 2–8°C. Do not freeze.
Running a different vial size or water volume? The DoseIQ calculator works it out for any combination — mcg, mg or IU, on any syringe size.
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This page is an educational research reference and is not medical advice. FOXO4-DRI is not approved for human use in any jurisdiction. Dose figures reflect the published record and what the community has converged on — starting points to weigh, not instructions. Consult a qualified healthcare provider before starting any protocol.